DNA and Protein Analysis by Electrophoresis
We develop and perform DNA and protein electrophoresis assays for the separation, visualization, and evaluation of DNA, RNA, and proteins, generating valuable information on identity, purity, integrity, and analytical control.
- DNA, RNA, and protein electrophoresis
- Assessment of identity, purity, and integrity
- Qualitative and semi-quantitative analysis
- Support for quality control and analytical development
Electrophoresis enables sample characterization and product verification through robust methodologies tailored to the type of analyte and the objective of the study.
Its value lies in providing a direct evaluation of apparent size, integrity, purity, and consistency between samples or experimental conditions, with additional support for complementary assays.
We provide a simple, reproducible, and visually interpretable analytical readout of essential attributes of DNA, RNA, and proteins, with practical applications in development, quality control, and initial characterization.
What is electrophoresis and what is it used for?
Electrophoresis is a separation technique that allows the analysis of biological molecules based on their size and physicochemical properties.
It is used to:
- Evaluate DNA integrity
- Analyze protein purity
- Detect degradation or fragmentation
- Confirm protein identity
The challenge in biological analysis
Without a robust methodology, inconsistent or inconclusive results may arise. Therefore, biological products require:
- High-resolution separation
- Sensitivity to detect impurities
- Reproducible methods across batches
- Reliable interpretation of results
Advanced and standardized DNA and protein electrophoresis
At Netpharmalab, we develop all analyses using validated SOPs and full traceability. We implement optimized techniques that allow:
- Clear band separation
- Qualitative and semi-quantitative analysis
- Densitometric quantification
- Specific identification through immunodetection
What does our DNA and protein electrophoresis service include?
1. DNA Electrophoresis
Separation and visualization of nucleic acids using agarose gel, enabling:
- Evaluation of DNA integrity and size
- Detection of degradation
- Analysis of residual DNA (host or plasmid)
Applications:
- Residual DNA control in biologics
- Purification monitoring
- Process comparability
2. SDS-PAGE / Native PAGE for Proteins
Protein separation using polyacrylamide gel electrophoresis:
- SDS-PAGE → denatured proteins
- Native PAGE → proteins in functional state
This allows:
- Evaluation of relative purity
- Detection of aggregates and fragments
- Analysis of protein profiles
Applications:
- Monoclonal antibodies
- Recombinant proteins
- Stability and stress studies
3. Western Blot
Technique based on SDS-PAGE + membrane transfer + specific immunodetection, enabling:
- Confirmation of protein identity
- Detection of specific impurities
- Verification of biomarker expression
Electrophoresis Methodology
DNA Electrophoresis (agarose)
- Separation by size
- Visualization using fluorescent dyes
- Evaluation of integrity and degradation
SDS-PAGE / Native PAGE
- Separation by molecular weight or native state
- Staining (Coomassie, silver, fluorescence)
- Identification of protein variants
Western Blot
- Transfer to membrane
- Specific immunodetection
- Confirmation of target proteins
Assay Phases
- Sample preparation
- Gel run
- Development / transfer
- Analysis and interpretation
– Densitometry
– Comparison with standards
– Technical report
Regulatory Compliance
Our assays comply with:
- GxP approach
- Qualified equipment (IQ/OQ)
- Standardized SOPs
- Data and image traceability
Frequently Asked Questions (FAQ)
- What is the difference between SDS-PAGE and Native PAGE?: SDS-PAGE separates denatured proteins by molecular weight, while Native PAGE preserves their functional structure.
- What is Western Blot used for?: It allows identification of specific proteins using antibodies, confirming their identity.
- Can residual DNA in biologics be analyzed?: Yes, agarose electrophoresis is a key technique for evaluating residual or plasmid DNA.
- What types of products are analyzed?: Monoclonal antibodies, recombinant proteins, viral vectors, and other biologics.
- Are these assays suitable for quality control?: Yes, they are essential in quality control, development, and comparability of biological products.
Request more information about our biological product analysis services
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